![]() We will evaluate histology and markers of kidney function to determine the therapeutic effects on diabetic nephropathy. In Aim 2, we hypothesize that the exosomes will reduce podocyte dysfunction in diabetic mouse kidneys. In Aim 1, we hypothesize that the induced nephron progenitor cell-derived exosomes will protect human podocytes from apoptosis induced by high glucose by examining morphology, viability, and markers of apoptosis. We found that treatment with high glucose induced actin rearrangement and apoptosis. Our lab has successfully derived podocytes from human induced pluripotent stem cells using an accelerated method. This proposal will evaluate the potential of induced nephron progenitor exosomes to treat diabetic kidney disease. Diabetic nephropathy (DN) results from glomerular podocyte dysfunction in response to a high glucose environment. We have isolated and characterized exosomes from these induced nephron progenitor cells and find them to be abundant and protective in other settings. The reprogramming factors are SNAI2, EYA1, and SIX1. Induced nephron progenitors are derived from adult human cells in tissue culture through transcription factor reprogramming with inducible piggyBac transposons. Induced nephron progenitor cells mimic the nephron progenitor cells present only in the fetal kidney that go on to form most cell types of the adult nephron. Induced nephron progenitor-derived exosomes for diabetic nephropathy This study will provide preliminary data for future fully-powered SB reduction studies. Implemented into the T2D patients' daily routine and offer cardiometabolic benefits. Our intervention is significant because sedentary breaks can be easily and safely ![]() We will also evaluate whether our intervention can reduce SB (primary outcome) and improve physical activity, cardiometabolic and patient-centered outcomes (secondary outcomes). This proposal will evaluate the feasibility and acceptability of the SB reduction intervention. The control group (n=35) will be given the American Diabetes Association (ADA) booklet, “Taking Care of Type 2 Diabetes”. Our 12-week intervention (n=35) will consist of 1) an instructional/goal-setting session, 2) an activity tracker (Fitbit) providing stand/walk prompts, 3) a smart water bottle (HidrateSpark) that syncs with the Fitbit, and 4) weekly tailored text messages for behavior reinforcement and weekly goal monitoring. We proposed a pilot randomized control trial to develop a SB-reduction intervention focused on breaking sedentary time with standing/walking in CAD patients (n=70). However, little is known about the feasibility and preliminary efficacy of a technology-based SB reduction program for T2D patients in a free-living setting. In addition, the use of a smart water bottle may be a promising strategy to naturally break sedentary time due to the impact of increased water intake on kitchen and restroom breaks. Wearable technology can prompt users to take breaks from ![]() Reducing SB time with frequent standing/walking breaks is safe and offers cardiometabolic benefits. There is a pressing need to develop novel strategies to reduce SB and improve daily activity. Patients with T2D are particularly prone to high levels of SB (10-12 hours/day) and have a greater risk for the negative consequences of SB due to their low activity levels. Sedentary behavior (SB) is a strong modifiable risk factor for developing type 2 diabetes (T2D). Reducing Sedentary Time in Patients with Type 2 Diabetes: A Pilot Randomized Control Trial These P&F awards are supported by the Vanderbilt Diabetes Research Center (NIH grant DK20593). Vanderbilt Diabetes Center Discovery Program GrantĬovers diabetes and/or obesity-related pilot studies that utilize high-throughput facility BioVU and/or the mass spectrometry research centers Vanderbilt Diabetes Research Center P&F GrantĬovers basic and/or clinic research related to diabetes, metabolism, and/or obesity VDRC will be offering Pilot and Feasibility (P&F) Awards for 2023 in the following areas:
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